Key features and details | |
Cat. No. | MABL-2153 |
Name | Anti-Integrin alpha V mAbs |
Clone No. | AFD-17E 6 |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | FACS, immunoprecipitation, ELISA, FC |
Species Reactivity | Human |
Basic Information | |
Specificity | The antibody reacts with extracellular domains on human αv-integrin chains. |
Alternative Name | 272-17E6; Vitronectin receptor; Vitronectin receptor subunit alpha; CD antigen: CD51; Integrin alpha-V heavy chain; Integrin alpha-V light chain |
UniProt | P06756 |
Immunogen | The original antibody was generated by immunizing BALB/c mice with of purified placental αvβ3. |
Application Notes | αv-Integrin is an integral membrane protein involved in cell adhesion. Antibody screening by ELISA revealed the antibody reacted specifically with αvβ3. In ELISA on fixed cells (‘CELISA’) the antibody showed a reaction pattern consistent with its recognition of the αv integrin chain (cells expressing αvβ3 and αvβ5 (M21)). The ability of the antibody to recognize native αv integrins was tested by FACS analysis, where the αv-expressing cells (M21) reacted strongly with the antibody. Immunoprecipitation from surface labelled cells showed the antibody detected αv integrin in M21 cells. Furthermore, αv-integrins can function as receptors for vitronectin. However, the antibody had the ability to disrupt stable interaction between vitronectin and αvβ3, and blocked the growth of M21 tumors in nude mice. |
Antibody First Published | Mitjans et al. An anti-alpha v-integrin antibody that blocks integrin function inhibits the development of a human melanoma in nude mice. J Cell Sci. 1995 Aug;108 ( Pt 8):2825-38. PMID:7593323 |
Note on publication | The authors describe the generation of an antibody against purified human αvβ3 integrin |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
