Key features and details | |
Cat. No. | MABL-2143 |
Name | Anti-Insulin receptor mAbs |
Clone No. | AFD- BBBB138 |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | in vitro, in vivo, SPR, ELISA |
Species Reactivity | Human |
Basic Information | |
Specificity | The antibody is specific for the insulin receptor. The antibody recognises both short isoform (IR- A) and a long isoform (IR-B). The epitope of the antibody does not overlap with the insulin binding site. |
Alternative Name | IR |
UniProt | P06213 |
Immunogen | The original antibody was isolated from a human phage display library by panning against IR. |
Application Notes | The specificity of the antibody for IR was determined by ELISA analysis. The binding affinity of the antibody to IR-A and IR-B was measured by surface plasmon resonance (Kd= 2.57 and 2.98 nM respectively). The antibody blocked insulin induced IR phosphorylation in a dose-dependent manner on HuH7 cells. The antibody effectively decreased insulin induced glucose uptake at concentrations of 1 nM and 10 nM in vitro. The antibody induced hyperglycemia in C57 mice in in vivo experiments. |
Antibody First Published | |
Note on publication | |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
