Key features and details | |
Cat. No. | MABL-2142 |
Name | Anti-insulin receptor mAbs |
Clone No. | AFD- 83-14 |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | fluorescence microscopy, in vitro, in vivo, IP, WB, ELISA, FC, IHC |
Species Reactivity | Bovine, Pig, Sheep, Human, Rhesus Monkey |
Basic Information | |
Specificity | This antibody is specific for the extracellular domain of the human insulin receptor (HIR). Specifically, it recognizes amino acids 469 - 592 (exons 7 and 8) of the insulin receptor alpha subunit. Low to moderate cross-reaction with bovine IR was observed. This antibody also binds to the Rhesus monkey, pig, and sheep IR. |
Alternative Name | CD220; Insulin Receptor alpha; HHF5; IR; HIR |
UniProt | P06213 |
Immunogen | The original antibody was generated by immunizing Balb/c mice with IM-9 lymphocytes followed by purified human insulin receptor. |
Application Notes | The original format of this antibody (mouse IgG2a) was generated, and its binding to the HIR was characterized via ELISA, Western blot, and immunoprecipitation assays. Its epitope was characterized through competition/inhibitory experiments (Soos et al., 1986; PMID: 2427071). This antibody was used to study the internalization of the HIR (Paccaud et al., 1992; PMID: 1618809), demonstrating that it facilitated constitutive internalization of the HIR via clathrin-coated pits, independent of receptor kinase activation or autophosphorylation. This process reflected the natural homeostasis of HIR on the cell surface and was mediated by the receptor's interaction with the clathrin machinery. The Fab format of the antibody was created and exhibited the same internalization rates as the full antibody, confirming that the process was not influenced by inter- or intramolecular cross-linking (Paccaud et al., 1992; PMID: 1618809). In an in vitro setting, a radiolabeled 125I version of this antibody strongly bound the Rhesus monkey brain capillary insulin receptor in an IHC assay. It demonstrated an ED50 of 0.09 µg/ml (0.60 nM) toward isolated human brain capillaries from autopsy, with a calculated KD of 0.45 ± 0.10 nM. |
Antibody First Published | Soos et al. Monoclonal antibodies reacting with multiple epitopes on the human insulin receptor. Biochem J. 1986 Apr 1;235(1):199-208. PMID:2427071 |
Note on publication | The original publication describes the immunization of mice with IM-9 an purified human insulin receptor and the identification of the insulin receptor alpha extracellular domain epitope. |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
