Key features and details | |
Cat. No. | MABL-2093 |
Name | Anti-IL-6 mAbs |
Clone No. | AFD- 61H7 |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | ELISA |
Species Reactivity | Human |
Basic Information | |
Specificity | This antibody recognizes and binds interleukin-6 on the top of the four-helix bundle near the N and C termini. Here the VL domain sterically interferes with the C-terminal domain of IL-6R (residues 195–295). The overlap of the 61H7 epitope and the site I surface concentrates around a cavity occupied by both the HCDR3 loop of 61H7 and the IL-6R molecule. |
Alternative Name | IL6; IFNB2; BSF-2; CDF; Interleukin-6; IL-6; B-cell stimulatory factor 2; CTL differentiation factor; Hybridoma growth factor; Interferon beta-2; IFN-beta-2 |
UniProt | P05231 |
Immunogen | The original antibody was generated by immunizations of Llama glama with recombinant IL-6 followed by construction of phage display library, selection, screeing and purification. |
Application Notes | This antibody is recommended for identification of IL-6 in the sample. The ability of this antibody to compete with biotinylated IL-6 for binding to captured IL-6R was investigated using competitive ELISA. The in-vitro potency of the antibody was determined using a cell-based neutralizing bioassay using the B9 and 7TD1 cell line, which require IL-6 for survival and proliferation and respond to very low concentration of IL-6 (PMID: 27129274). |
Antibody First Published | Blanchetot et al. Structural Mimicry of Receptor Interaction by Antagonistic Interleukin-6 (IL-6) Antibodies. J Biol Chem. (2016); 291(26); 13846-54. PMID:27129274 |
Note on publication | Describes the crystal structure and epitope characterization of the antibody. |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
