Key features and details | |
Cat. No. | MABL-2080 |
Name | Anti-IL-4 mAbs |
Clone No. | AFD- hu3B9 |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | in vivo, inhibition, ELISA |
Species Reactivity | Human |
Basic Information | |
Specificity | This antibody is specific for human IL-4 and binds a non-contiguous epitope. |
Alternative Name | IL4; BSF-1; Interleukin-4; B-cell stimulatory factor 1; Binetrakin; Lymphocyte stimulatory factor 1; Pitrakinra; 3B9 |
UniProt | P05112 |
Immunogen | The original antibody was generate by immunizing F1 hybrids of Balb/c and C57BL/6 with E. Coli-produced recombinant human IL-4. |
Application Notes | The binding affinity of the original format and Fab fragment of this antibody were evaluated by ELISA. The original format of this antibody (mouse IgG1) exhibited a 0.2 nM Kd (~0.18 nM). The Kd of its Fab fragment was 0.3 nM. This antibody successfully inhibited IL-4 binding to its receptor, as evaluated using 125 I-rhIL4 binding to the gibbon cell line, MLA. This antibody successfully inhibited lymphocyte proliferation — the original format of this antibody inhibited 3 H-thymidine incorporation by human peripheral blood T lymphocytes stimulated with 133 pM IL-4 and human tonsillar B lymphocytes stimulated by 167 pM IL-4. The IC50 for inhibition of T cell proliferation was 30 pM, and for B cell proliferation 103 pM. The corresponding values for the Fab fragment were 108 and 393 pM. This antibody successfully inhibited CD23 induction — the original format of this antibody inhibited CD23 expression on human tonsil B lymphocytes stimulated with 8.3 pM IL-4 with an IC50 value of 136 pM. |
Antibody First Published | |
Note on publication | The original publication describes the generation and characterization of various anti-human IL-4 antibodies and their derivatives, including its humanization. |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
