Key features and details | |
Cat. No. | MABL-2077 |
Name | Anti-IL-4 mAbs |
Clone No. | AFD- 25D2-9 |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | inhibit, ELISA |
Species Reactivity | Human |
Basic Information | |
Specificity | This antibody binds human IL-4. Interleukin-4 is a cytokine secreted primarily by mast cells, T- cells, eosinophils, and basophils that plays a role in regulating antibody production, hematopoiesis and inflammation, and the development of effector T-cell responses. It induces differentiation of naive helper T cells (Th0 cells) to Th2 cells. It also induces expression of class II MHC molecules on resting B-cells and enhances both secretion and cell surface expression of IgE and IgG1. |
Alternative Name | IL4; BSF-1; Interleukin-4; B-cell stimulatory factor 1; Binetrakin; Lymphocyte stimulatory factor 1; Pitrakinra; MP4.25D2.11; ATCC HB 9809 |
UniProt | P05112 |
Immunogen | The original antibody was generated by immunizing Lewis rat with 1ml of human IL-4. The humanized version of the antibody was created later by grafting CDRs from the original rat antibody onto human framework regions. |
Application Notes | The binding characterization of this antibody for human IL-4 was done using ELISA. This antibody was also capable of inhibiting binding of radiolabeled hIL-4 to recombinant hIL-4 receptors expressed on the Jijoye CJ cell line. This antibody was also reported to inhibit induction of human growth hormone in the germline epsilon reporter assay (US5597710; US5863537). |
Antibody First Published | |
Note on publication | |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
