Key features and details | |
Cat. No. | MABL-2072 |
Name | Anti-IL-34 mAbs |
Clone No. | AFD- FAb1.1 |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | Blocking, crystallization, neutralizing |
Species Reactivity | Human, Mouse |
Basic Information | |
Specificity | The antibody is specific for human and murine IL-34. The antibody recognizes a largely continuous area at the junction of two IL-34 protomers. The epitope overlaps largely with binding epitopes responsible for CSF-1R binding. |
Alternative Name | Interleukin-34; YW404.33.56 |
UniProt | Q6ZMJ4 |
Immunogen | The original antibody was generated by panning a Fab library against IL-34. |
Application Notes | The crystal structure of the Fab fragment of the antibody in complex with human IL-34 was determined. The original antibody was reformatted as an IgG molecules and the binding affinity of both formats measured by BLI experiments (Kd= 17 nM and 120 pM respectively). The antibody blocked the biological activity of IL-34 in human monocyte viability. The antibody neutralized IL-34 in a proliferation assays (Ma et al., 2012; PMID: 22483114). |
Antibody First Published | Ma et al. Structural basis for the dual recognition of helical cytokines IL-34 and CSF-1 by CSF-1R. Structure. 2012 Apr 4;20(4):676-87. PMID:22483114 |
Note on publication | The original paper shows the structure of IL-34 in complex with the Fab fragment of the antibody. |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
