Anti-IL-21R mAbs [ATR-107](MABL-2067)

Cat. No.

MABL-2067

Name

Anti-IL-21R mAbs

Clone No.

AFD- ATR-107

From

Recombinant Antibody

Isotype

Engineer antibody

Application

neutralising, therapeutic

Species Reactivity

Human, Cynomolgus Monkey, Mouse

Basic Information

Specificity

This antibody recognises both human and   murine IL-21R extracellular domains, as both were used as targets during   alternating rounds of phage display library panning. This antibody also binds  to rat and cynomolgus monkey IL-21R, but does not cross-react with human   IL-4Rα or IL-2Rβ.

Alternative Name

CD360; Ab01; Ab-01; ATR107; IL21R;   Interleukin-21 receptor; IL-21 receptor; NILR; Novel interleukin receptor;   Q9JHX3

UniProt

Q9HBE5

Immunogen

This antibody was isolated through phage   display. The scFv parental clone (18A5), which binds human IL-21R, was   isolated from the CS human scFv library, using BaF3 cells expressing human   IL- 21R and biotinylated IL-21R-Fc fusion protein. Four phage display   libraries were constructed based on 18A5, and variants with increased   affinity for both human and murine IL-21R were identified, including ATR-107.

Application Notes

This antibody binds to both human and murine   IL-21R; surface plasmon resonance analysis has revealed this antibody to have   an affinity to human and cynomolgus monkey IL-21R of 2 nM, and to murine   IL-21R of 16 nM (Vugmeyster et al, 2010). This antibody has been shown to   neutralise the IL- 21-dependent proliferation of both activated human primary   B and CD4+ T cells and mouse CD8+ T cells (Vugmeyster et al, 2010).   Additionally, this antibody inhibits IL-21-induced phosphorylation of STAT3   in CD4+ T cells in a concentration-dependent manner (Zhu et al, 2013). In the   MRL-Faslpr mouse model of lupus, administration of this antibody has been   shown to reduce anti-dsDNA antibodies and IgG deposits in the kidney   (Vugmeyster et al, 2010). This antibody did not induce cytokine   storm-associated gene activation in human PBMCs in vitro, nor when   administered to cynomolgus monkeys (Guo et al, 2010). 

Antibody First   Published

Vugmeyster et al. In vitro potency,   pharmacokinetic profiles and pharmacological activity of optimized   anti-IL-21R antibodies in a mouse model of lupus MAbs. 2010 May-Jun; 2(3)： 335–346. PMID：20424514

Note on publication

Describes the original generation and   characterisation of this antibody.

COA Information （For reference only, actual COA shall prevail）

Size

100 μg Purified   antibody.

Concentration

1 mg/ml.

Purification

Protein A affinity   purified

Buffer

PBS with 0.02% Proclin   300.

Concentration

1 mg/ml.

Storage   Recommendation

Store at 4⁰C for up to 3   months. For longer storage, aliquot and store at - 20⁰C.