Key features and details | |
Cat. No. | MABL-2067 |
Name | Anti-IL-21R mAbs |
Clone No. | AFD- ATR-107 |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | neutralising, therapeutic |
Species Reactivity | Human, Cynomolgus Monkey, Mouse |
Basic Information | |
Specificity | This antibody recognises both human and murine IL-21R extracellular domains, as both were used as targets during alternating rounds of phage display library panning. This antibody also binds to rat and cynomolgus monkey IL-21R, but does not cross-react with human IL-4Rα or IL-2Rβ. |
Alternative Name | CD360; Ab01; Ab-01; ATR107; IL21R; Interleukin-21 receptor; IL-21 receptor; NILR; Novel interleukin receptor; Q9JHX3 |
UniProt | Q9HBE5 |
Immunogen | This antibody was isolated through phage display. The scFv parental clone (18A5), which binds human IL-21R, was isolated from the CS human scFv library, using BaF3 cells expressing human IL- 21R and biotinylated IL-21R-Fc fusion protein. Four phage display libraries were constructed based on 18A5, and variants with increased affinity for both human and murine IL-21R were identified, including ATR-107. |
Application Notes | This antibody binds to both human and murine IL-21R; surface plasmon resonance analysis has revealed this antibody to have an affinity to human and cynomolgus monkey IL-21R of 2 nM, and to murine IL-21R of 16 nM (Vugmeyster et al, 2010). This antibody has been shown to neutralise the IL- 21-dependent proliferation of both activated human primary B and CD4+ T cells and mouse CD8+ T cells (Vugmeyster et al, 2010). Additionally, this antibody inhibits IL-21-induced phosphorylation of STAT3 in CD4+ T cells in a concentration-dependent manner (Zhu et al, 2013). In the MRL-Faslpr mouse model of lupus, administration of this antibody has been shown to reduce anti-dsDNA antibodies and IgG deposits in the kidney (Vugmeyster et al, 2010). This antibody did not induce cytokine storm-associated gene activation in human PBMCs in vitro, nor when administered to cynomolgus monkeys (Guo et al, 2010). |
Antibody First Published | Vugmeyster et al. In vitro potency, pharmacokinetic profiles and pharmacological activity of optimized anti-IL-21R antibodies in a mouse model of lupus MAbs. 2010 May-Jun; 2(3)： 335–346. PMID：20424514 |
Note on publication | Describes the original generation and characterisation of this antibody. |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
