Key features and details | |
Cat. No. | MABL-2060 |
Name | Anti-IL-2 mAbs |
Clone No. | AFD- NARA1 |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | crystallography, in vitro, in vivo, SPR, FC |
Species Reactivity | Human |
Basic Information | |
Specificity | The antibody is specific for human IL-2. The antibody binds to a three-dimensional epitope of hIL-2 (residues P34, K35, R38, T41, Q74, and K76). The antibody overlaps with the CD25-binding epitope of IL-2. The antibody does not cross reacts with mouse IL-2. |
Alternative Name | IL2; IL-2; TCGF; Interleukin-2; T-cell growth factor; Aldesleukin |
UniProt | P60568 |
Immunogen | The original antibody was generated immunizing Balb/c mice with human IL-2. |
Application Notes | SPR showed the binding of the antibody to IL-2 prevented further binding of hCD25 to IL-2; the affinity of the antibody to hIL-2 was ~10−9 M. The crystal structure of the Fab fragment in complex with IL-2 was determined. IL-2/antibody complexes preferentially expand CD8+ T and NK cells in vivo while disfavoring CD25+ Tregs in transplantable metastatic melanoma model. This difference was also confirmed by kinetic proliferation studies. The complex IL-2/antibody decreased the tumor growth in mice by increasing the CD8+ T cell responses. The IL-2/antibody complex immunotherapy was also efficacious in a spontaneous melanoma model in mice. The complex Il-2/antibody shows potential as a therapeutic agent as it achieved strong antitumor immune responses (Arenas-Ramirez et al., 2016; PMID: 27903862). In order to prevent in vivo dissociation of the complex IL-2/antibody, NARA1leukin was constructed by integrating IL-2 into the antigen-binding groove on the antibody. |
Antibody First Published | Ramirez et al. Improved cancer immunotherapy by a CD25-mimobody conferring selectivity to human interleukin-2. Sci Transl Med. 2016 Nov 30;8(367):367ra166.PMID:27903862 |
Note on publication | The paper describes the generation and characterization of the antibody, which is specific for IL-2 and it overlaps with the CD25-binding epitope of IL-2 |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
