Key features and details | |
Cat. No. | MABL-2043 |
Name | Anti-IL-18 mAbs |
Clone No. | AFD- ABT-325 (A-794722.0; 2.5(E)) |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | IP, neutralize, ELISA, FC |
Species Reactivity | Human, Cynomolgus Monkey |
Basic Information | |
Specificity | This antibody binds human and cynomolgus monkey IL-18 and does not cross react with mouse, rat or dog IL-18. Interleukin-18 is a pro-inflammatory cytokine primarily involved in epithelial barrier repair, polarized T-helper 1 (Th1) cell and natural killer (NK) cell immune responses. |
Alternative Name | Interleukin-18; IL18; IGIF; IL1F4; Iboctadekin; Interferon gamma-inducing factor; IFN-gamma-inducing factor; Interleukin-1 gamma; IL-1 gamma; ABT325; 2.5(E)mg1 |
UniProt | Q14116 |
Immunogen | The original antibody was generated by immunizing transgenic Xenomouse with human IL-18 in phosphate-buffered saline. |
Application Notes | The binding characterization of this antibody to human and cynomolgus monkey IL-18 was done using BIACORE assay. The binding affinity of the human IgG1 version was reported to be Kd=0.196 nM. This antibody can be used to immunoprecipitate human and cynomolgus monkey IL-18. This antibody neutralized both recombinant (KG-1 and RBA) and natural IL-18 (WBA) in an in vitro assay. The ability of this antibody to neutralize natural human IL-18-induced IFNγ in an inflammatory environment in vivo, the severe combined immunodeficient (SCID) mouse model was used wherein human PBMCs were injected into the mouse and the cells were stimulated in vivo to produce human IL-18 (HuPBMC-SCID model). This antibody inhibited human IL-18-dependent human IFNγ production in vivo with a clear dose- response by either route of administration. The binding of C1q to this antibody was determined using standard ELISA techniques (US20050100965). |
Antibody First Published | |
Note on publication | |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
