Key features and details | |
Cat. No. | MABL-2030 |
Name | Anti-IL-13 mAbs |
Clone No. | AFD- M1295 |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | neutralize, therapeutic, ELISA |
Species Reactivity | Human |
Basic Information | |
Specificity | This antibody binds human interleukin-13 at the surface of helices A and D close to N- and C- termini of the IL-13 polypeptide chain. IL-13 is a cytokine secreted by T helper type 2 (Th2) cells, CD4 cells, natural killer T cell, mast cells, basophils, eosinophils and nuocytes. It inhibits the production of inflammatory cytokines (IL-1, IL-6, TNF, IL-8) by LPS-stimulated monocytes. It synergizes with IL2 in regulating interferon-gamma synthesis. It is a mediator of allergic inflammation and different diseases including asthma. |
Alternative Name | IL13; NC30; Interleukin-13; hC836; m836; H2L6; LC6HC2; HFAL62 |
UniProt | P35225 |
Immunogen | The original mouse antibody m836 was generated by immunizing BALB/c mice with a natural variant of human IL-13 known as R130Q. The humanized version H2L6 was generated by grafting CDRs of mouse antibody onto selected human heavy chain and light chain frameworks and finally site-directed mutagenesis of two methionine in the CDRs of heavy chain with binding affinity optimization resulted in the generated of M1295. |
Application Notes | This antibody can bind human IL13 in an ELISA. The affinity of original IgG1 antibody for wild type human IL-13 was determined by BIAcore to be 5.7 ± 1.3 pM (PMID: 20226193). Preclinical studies confirm the functional effects of M1295-mediated neutralization of human IL-13 in human cell based in vitro assays using mechanistically relevant endpoints associated with the pathogenesis of asthma, atopic dermatitis, and eosinophil-mediated disease (US8399630). |
Antibody First Published | Fransson et al. Human framework adaptation of a mouse anti-human IL-13 antibody. J Mol Biol. 2010 Apr 30;398(2):214-31. PMID:20226193 |
Note on publication | Describes the humanization and site directed affinity maturation of an anti-IL13 antibody. |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
