Key features and details | |
Cat. No. | MABL-2022 |
Name | Anti-IL-12 mAbs |
Clone No. | AFD- 7G11 |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | ELISA |
Species Reactivity | Human |
Basic Information | |
Specificity | This antibody binds human interleukin 12. It is required for early control of infection and for generation maintenance of acquired protective immunity. |
Alternative Name | IL12; IL-12p70; IL12p70 |
UniProt | P29459; P29460 |
Immunogen | The original antibody was generated by immunizing New Zealand rabbits with recombinant human IL-12 protein containing Ile23-Ser328 sequence of IL-12A and Arg23-Ser219 sequence of IL-12B. |
Application Notes | The original rabbit IgG antibody binds human recombinant interleukin-12 protein containing Ile23-Ser328 sequence of IL-12A and Arg23-Ser219 sequence of IL-12B with a binding affinity of Kd=2.14 nM. A combination of 12E3 and 7G11 can be used in a double antibody sandwich ELISA, where 12E3 is used as a capture antibody and biotin-labeled 7G11 is used as a detection antibody. The sensitivity of human IL-12 detection for this pair was reported to be 11.05pg/mL (11.05ng/L) (CN115819580). |
Antibody First Published | |
Note on publication | |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
