Key features and details | |
Cat. No. | MABL-1974 |
Name | Anti-IgG1 (hinge) mAbs |
Clone No. | AFD- TP885 |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | dot blot assay, IP |
Species Reactivity | Rat, Mouse |
Basic Information | |
Specificity | This antibody recognises mouse and rat IgG1, with a proposed epitope located at the hinge region. |
Alternative Name | immunoglobulin G1 |
UniProt | |
Immunogen | This antibody was raised by immunising alpacas with 1.0 mg polyclonal mouse IgG. Subsequently, the generation of nanobody immune libraries and the selection of antigen-specific nanobodies by phage display from these libraries were performed. |
Application Notes | TP885 nanobody can be used for the purification of antigens or antigen complexes previously bound to a primary antibody, as it successfully isolated mouse monoclonal IgG1 from hybridoma cell culture supernatant and then released it under physiological conditions using SUMOStar protease cleavage (Pleiner et al., 2018). |
Antibody First Published | Pleiner et al. A toolbox of anti–mouse and anti–rabbit IgG secondary nanobodies. J Cell Biol. 2018 Mar 5;217(3):1143-1154. doi: 10.1083/jcb.201709115. Epub 2017 Dec 20. PMID:29263082 |
Note on publication | The article describes the generation and characterisation of this nanobody. |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
