Anti-IgG-Fc Domain mAbs

Cat. No.

MABL-1973

Name

Anti-IgG-Fc Domain mAbs

Clone No.

AFD- IgG-Fc-16

From

Recombinant Antibody

Isotype

Engineer antibody

Application

SPR, ELISA

Species Reactivity

Bovine, Cat, chimpanzee, Dog, Donkey, Goat,   Guinea Pig, Horse, Rabbit, Rat, rhesus macaque, Sheep, Swine, Syrian Hamster,   Human, Mouse

Basic Information

Specificity

This antibody is specific for the mammalian IgG-Fc domain. It was tested for and found to bind the human IgG-Fc domain (all subclasses). In a cross-reactivity test, it also bound bovine, mouse,   rat, rabbit, dog, cat, swine, sheep, Chimpanzee, Rhesus macaque, donkey,   horse, goat, Syrian hamster, and guinea pig IgG-Fcs.

Alternative Name

UniProt

Immunogen

The original antibody was generated by immunizing   llamas with mammalian IgG antibodies and Fc fragments, creating the   appropriate phage display library, and panning against the original target.

Application Notes

The reactivity of the original version of this antibody (camelid (llama) VHH) was tested using ELISA. Its binding   affinity (KD) was measured using SPR and was found to be 13.1, 6.81, 16.2,  21.3, 1.70, 2.79, and 8.41 nM for human IgG, human IgG1, human IgG2, human   IgG3, human IgG4, bovine IgG, and mouse IgG, respectively. Its binding to   human, mouse, and bovine IgG was further verified via chromatography testing;   the dynamic binding capacity of the antibody when immobilized onto NHS   sepharose was 11.5 mg human IgG/ml matrix (US9040666B2).

Antibody First   Published

Note on publication

COA Information （For reference only, actual COA shall prevail）

Size

100 μg Purified   antibody.

Concentration

1 mg/ml.

Purification

Protein A affinity   purified

Buffer

PBS with 0.02% Proclin   300.

Concentration

1 mg/ml.

Storage Recommendation

Store at 4⁰C for up to 3   months. For longer storage, aliquot and store at - 20⁰C.