Key features and details | |
Cat. No. | MABL-1950 |
Name | Anti-IgE mAbs |
Clone No. | AFD-rhuMAb-E25 |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | Blocking, in vitro, in vivo, therapeutic |
Species Reactivity | Human |
Basic Information | |
Specificity | This antibody is specific for the Fc region of human IgE. |
Alternative Name | Immunoglobulin epsilon; FcERI; FCER1; FCϵRI; FCϵR1; Fc-epsilon RI-alpha; IgE Fc receptor subunit alpha |
UniProt | P12319 |
Immunogen | The original antibody is the recombinant humanized version of MaE11, a murine anti-IgE monoclonal antibody, constructed from the complementarity determining regions of MaE11 and a human IgG1 frame. |
Application Notes | This antibody has two potential binding sites on IgE (one for each Fc heavy chain) and, therefore, can function as a multivalent ligand that complexes with IgE in various conformations depending on the ratio of their molar concentrations (Liu et al., 1995; PMID: 7654701). This antibody has been shown to reduce serum IgE levels and significantly attenuate both the early- and late-phase responses to inhaled allergen in allergic asthmatic subjects (Fahy et al., 1997; PMID: 9196082). Omalizumab treatment has been shown to reduce asthma exacerbations and decrease inhaled corticosteroid and rescue medication use in the treatment of severe allergic asthma (Busse et al., 2001; PMID: 11496232). The therapeutic product made using this antibody (omalizumab) binds to human IgE in blood or membrane-bound IgE on B lymphocytes. Furthermore, omalizumab competes with IgE's high and low-affinity receptors — FcεRI and CD23, respectively — for binding to residues in the CH3 domain. |
Antibody First Published | Liu et al. Characterization of complex formation by humanized anti-IgE monoclonal antibody and monoclonal human IgE Biochemistry. 1995 Aug 22;34(33):10474-82. doi: 10.1021/bi00033a020 PMID:7654701 |
Note on publication | The original publication characterizes the formation of immune complexes between a humanized anti-IgE monoclonal antibody and IgE, revealing various complex structures and a high-affinity interaction. |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
