Key features and details | |
Cat. No. | MABL-1936 |
Name | Anti-IFN-gamma mAbs |
Clone No. | AFD- 1F5 |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | ELISA |
Species Reactivity | White rhinoceros (Ceratotherium simum) |
Basic Information | |
Specificity | scFv 1F5 reacted with both white rhino (Ceratotherium simum) and Asian elephant (Elephas maximus) IFN-gamma, shown by direct ELISAs. |
Alternative Name | IFN-g; Interferon gamma; Type II interferon; IFN-gamma; IFN-y |
UniProt | A0SDD7 |
Immunogen | Chicken antibodies were produced by immunising Hy-Line silver brown chickens with recombinant white rhino IFN-gamma. The spleens of immunised chickens were collected and total RNA was reverse transcribed into cDNA. |
Application Notes | 1F5 has been used as a capture antibody in ELISAs testing for the presence of IFN- gamma. |
Antibody First Published | Morar et al. Cloning, sequencing and expression of white rhinoceros (Ceratotherium simum) interferon-gamma (IFN-gamma) and the production of rhinoceros IFN-gamma specific antibodies. Vet Immunol Immunopathol. 2007 Jan 15;115(1-2):146-54. PMID:17118460 |
Note on publication | Describes the generation of murine and chicken antibodies against white rhino IFN- gamma and their use in the development of a diagnostic assay for the detection of tuberculosis. |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
