Key features and details | |
Cat. No. | MABL-1930 |
Name | Anti-IDH1 R132H mAbs |
Clone No. | AFD- 10G5-1 |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | ELISA, IHC |
Species Reactivity | Human |
Basic Information | |
Specificity | This antibody binds human isocitrate dehydrogenase 1 (IDH1) having R132H mutation. IDH1 is an enzyme that catalyzes the NADP+-dependent oxidative decarboxylation of isocitrate (D-threo-isocitrate) to 2-ketoglutarate (2-oxoglutarate), which is required by other enzymes such as the phytanoyl-CoA dioxygenase. It plays a critical role in the generation of NADPH, an important cofactor in many biosynthesis pathways. The NADPH produced from isocitrate dehydrogenase 1 is involved in the breakdown of fats for energy, and it also protects cells from potentially harmful molecules called reactive oxygen species. |
Alternative Name | IDH; IDH1; IDPc; Isocitrate dehydrogenase [NADP] cytoplasmic; Isocitrate dehydrogenase 1; Cytosolic NADP-isocitrate dehydrogenase; NADP(+)-specific ICDH; Oxalosuccinate decarboxylase |
UniProt | O75874 |
Immunogen | The original antibody was generated by immunizing BALB/c mice with human IDH-R132H polypeptide (KPIIGHHAYGDQYC) with an N-terminal BSA conjugation. |
Application Notes | The binding reactivity of this antibody to R132H mutant of human IDH1 was determined using ELISA. The original mouse IgG antibody binds IDH1 R132H mutant with an affinity of Kd= 5.76 X10 ^(-10) M. This antibody was used in the immunohistochemical analysis for detection of IDH1 R132H protein in cancerous tissues (CN108727500). |
Antibody First Published | |
Note on publication | |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
