Key features and details | |
Cat. No. | MABL-1929 |
Name | Anti-IDH1 mutant mAbs |
Clone No. | AFD- MsMab-1 |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | WB, ELISA, IHC |
Species Reactivity | Human |
Basic Information | |
Specificity | This antibody binds human isocitrate dehydrogenase 1 (IDH1) having R132H, R132C, R132S, R132G and R132L mutations and does not cross react with wild-type IDH1 protein. It also recognizes IDH2- R172M (GGTKPITIGMHAHGDQYKA) and IDH2-R172S (GGTKPITIGSHAHGDQYKA). IDH1 is an enzyme that catalyzes the NADP+-dependent oxidative decarboxylation of isocitrate (D-threo-isocitrate) to 2- ketoglutarate (2-oxoglutarate), which is required by other enzymes such as the phytanoyl-CoA dioxygenase. It plays a critical role in the generation of NADPH, an important cofactor in many biosynthesis pathways. |
Alternative Name | IDH; IDH1; IDPc; Isocitrate dehydrogenase [NADP] cytoplasmic; Isocitrate dehydrogenase 1; Cytosolic NADP-isocitrate dehydrogenase; NADP(+)-specific ICDH; Oxalosuccinate decarboxylase; IDH2; Isocitrate dehydrogenase [NADP]; mitochondrial; ICD-M; IDP; NADP(+)-specific ICDH; Oxalosuccinate decarboxylase |
UniProt | O75874; P48735 |
Immunogen | The original antibody was generated by immunizing BDF1 mice with synthetic IDH1-R132G peptide of 19 amino acids (GGVKPIIIGGHAYGDQYRA) conjugated with KLH. |
Application Notes | The binding reactivity of this antibody to wild-type IDH1 and its mutants (R132H, R132C, R132S, R132G and R132L) peptides was determined using ELISA. Western blot analysis also revealed that MsMab-1 reacted with recombinant proteins of IDH1-R132H, IDH1-R132S, and IDH1-R132G, but not with wild type IDH1 and other IDH1 mutations. This antibody also recognizes IDH2-R172M protein, despite that the IDH1-R132G peptide shows only 73.7% identity with the equivalent portion of IDH2-R172M (GGTKPITIGMHAHGDQYKA). This antibody successfully stained the IDH1-R132S or IDH1-R132G-expressing glioma cells in immunohistochemistry (PMID: 23782684). This antibody can bind IDH2-R172S mutant of IDH2 in an ELISA and can recognize PA-tagged IDH2-R172S recombinant protein in a western blot. This antibody was also used to stain IDH2-R172S-expressing osteosarcoma tissues in immunohistochemistry (PMID: 24403254). |
Antibody First Published | Kaneko et al. Establishment of a multi-specific monoclonal antibody MsMab- 1 recognizing both IDH1 and IDH2 mutations. Tohoku J Exp Med. 2013 Jun;230(2):103-9. PMID:23782684 |
Note on publication | Describes the generation of a multi-specific antibody that is capable of identifying both IDH1 and IDH2 mutations and can be used in the immunohistochemical determination of glioma cells. |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
