Anti-IDH1 mutant mAbs

Cat. No.

MABL-1929

Name

Anti-IDH1 mutant mAbs

Clone No.

AFD- MsMab-1

From

Recombinant Antibody

Isotype

Engineer antibody

Application

WB, ELISA, IHC

Species Reactivity

Human

Basic Information

Specificity

This antibody binds human isocitrate   dehydrogenase 1 (IDH1) having R132H, R132C, R132S, R132G and R132L mutations   and does not cross react with wild-type IDH1 protein. It also recognizes   IDH2- R172M (GGTKPITIGMHAHGDQYKA) and IDH2-R172S (GGTKPITIGSHAHGDQYKA). IDH1   is an enzyme that catalyzes the NADP+-dependent oxidative decarboxylation of   isocitrate (D-threo-isocitrate) to 2- ketoglutarate (2-oxoglutarate), which   is required by other enzymes such as the phytanoyl-CoA dioxygenase. It plays   a critical role in the generation of NADPH, an important cofactor in many   biosynthesis pathways. 

Alternative Name

IDH; IDH1; IDPc; Isocitrate dehydrogenase  [NADP] cytoplasmic; Isocitrate dehydrogenase 1; Cytosolic NADP-isocitrate dehydrogenase; NADP(+)-specific ICDH; Oxalosuccinate decarboxylase; IDH2;   Isocitrate dehydrogenase [NADP]; mitochondrial; ICD-M; IDP; NADP(+)-specific   ICDH; Oxalosuccinate decarboxylase

UniProt

O75874; P48735

Immunogen

The original antibody was generated by   immunizing BDF1 mice with synthetic IDH1-R132G peptide of 19 amino acids (GGVKPIIIGGHAYGDQYRA)   conjugated with KLH.

Application Notes

The binding reactivity of this antibody to   wild-type IDH1 and its mutants (R132H, R132C, R132S, R132G and R132L)   peptides was determined using ELISA. Western blot analysis also revealed that   MsMab-1 reacted with recombinant proteins of IDH1-R132H, IDH1-R132S, and IDH1-R132G, but not with wild type IDH1 and other IDH1 mutations. This antibody also recognizes IDH2-R172M protein, despite that the IDH1-R132G peptide shows only 73.7% identity with the equivalent portion of IDH2-R172M   (GGTKPITIGMHAHGDQYKA). This antibody successfully stained the IDH1-R132S or   IDH1-R132G-expressing glioma cells in immunohistochemistry (PMID: 23782684). This antibody can bind IDH2-R172S mutant of IDH2 in an ELISA and can   recognize PA-tagged IDH2-R172S recombinant protein in a western blot. This   antibody was also used to stain IDH2-R172S-expressing osteosarcoma tissues in immunohistochemistry (PMID: 24403254). 

Antibody First   Published

Kaneko et al. Establishment of a multi-specific monoclonal antibody MsMab- 1 recognizing both IDH1 and IDH2   mutations. Tohoku J Exp Med. 2013 Jun;230(2):103-9. PMID:23782684

Note on publication

Describes the generation of a multi-specific antibody that is capable of identifying both IDH1 and IDH2 mutations and can be used in the immunohistochemical determination of glioma cells.

COA Information （For reference only, actual COA shall prevail）

Size

100 μg Purified   antibody.

Concentration

1 mg/ml.

Purification

Protein A affinity   purified

Buffer

PBS with 0.02% Proclin   300.

Concentration

1 mg/ml.

Storage   Recommendation

Store at 4⁰C for up to 3   months. For longer storage, aliquot and store at - 20⁰C.