Anti-ICOS ligand mAbs

Cat. No.

MABL-1924

Name

Anti-ICOS ligand mAbs

Clone No.

AFD- Clone 323

From

Recombinant Antibody

Isotype

Engineer antibody

Application

inhibit, Block, ELISA, FC

Species Reactivity

Human

Basic Information

Specificity

This antibody binds human B7RP-1, which is a   ligand for T-cell-specific cell surface receptor ICOS. It acts as a   costimulatory signal for T-cell proliferation and cytokine secretion and also   induces B-cell proliferation and differentiation into plasma cells.

Alternative Name

CD275; ICOSLG; ICOSL; B7-H2; B7H2; B7RP-1;  B7RP1; KIAA0653; B7 homolog 2; B7-like protein GL50; B7-related protein 1

UniProt

O75144

Immunogen

The original antibody was isolated from a human naïve scFv phage library and panning against B7RP-1-Fc protein.

Application Notes

The scFv version of this antibody binds   B7RP-1-Fc with a Kd= 9nM. The binding specificity of this antibody was   confirmed using ELISA. This scFv version of this antibody was shown to bind   B7RP-1-Fc in an ELISA. This antibody recognized B7RP-1 molecules expressed on   B cells, as assessed by flow cytometry. This antibody is capable of   inhibiting interaction between ICOS-Fc and B7RP-1-Fc. This antibody also   exhibited inhibitory activity in a proliferation assay of T cells stimulated   with anti-CD3 mAb and B7RP-1-Fc (PMID: 20065650).

Antibody First   Published

Maeda et al. Regulation of T cell response by blocking the ICOS signal with the B7RP-1-specific small antibody fragment isolated from human antibody phage library. MAbs. (2009); 1(5): 453–461.   PMID:20065650

Note on publication

Describes the generation of this antibody   and its use in the study of T cell response by blocking the ICOS signal using   this antibody.

COA Information （For reference only, actual COA shall prevail）

Size

100 μg Purified   antibody.

Concentration

1 mg/ml.

Purification

Protein A affinity   purified

Buffer

PBS with 0.02% Proclin   300.

Concentration

1 mg/ml.

Storage   Recommendation

Store at 4⁰C for up to 3   months. For longer storage, aliquot and store at - 20⁰C.