Key features and details | |
Cat. No. | MABL-1923 |
Name | Anti-ICOS ligand mAbs |
Clone No. | AFD- Clone 223 |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | inhibit, Block, ELISA, FC |
Species Reactivity | Human |
Basic Information | |
Specificity | This antibody binds human B7RP-1, which is a ligand for T-cell-specific cell surface receptor ICOS. It acts as a costimulatory signal for T-cell proliferation and cytokine secretion and also induces B-cell proliferation and differentiation into plasma cells. |
Alternative Name | CD275; ICOSLG; ICOSL; B7-H2; B7H2; B7RP-1; B7RP1; KIAA0653; B7 homolog 2; B7-like protein GL50; B7-related protein 1 |
UniProt | O75144 |
Immunogen | The original antibody was isolated from a human naïve scFv phage library and panning against B7RP-1-Fc protein. |
Application Notes | The scFv version of this antibody binds B7RP-1-Fc with a Kd= 14nM. The binding specificity of this antibody was confirmed using ELISA. This scFv version of this antibody was shown to bind B7RP-1-Fc in an ELISA. This antibody recognized B7RP-1 molecules expressed on B cells, as assessed by flow cytometry. This antibody is capable of inhibiting interaction between ICOS-Fc and B7RP-1-Fc. This antibody also exhibited inhibitory activity in a proliferation assay of T cells stimulated with anti-CD3 mAb and B7RP-1-Fc (PMID:20065650). |
Antibody First Published | Maeda et al. Regulation of T cell response by blocking the ICOS signal with the B7RP-1-specific small antibody fragment isolated from human antibody phage library. MAbs. (2009); 1(5): 453–461. PMID:20065650 |
Note on publication | Describes the generation of this antibody and its use in the study of T cell response by blocking the ICOS signal using this antibody. |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
