Key features and details | |
Cat. No. | MABL-1921 |
Name | Anti-ICOS mAbs |
Clone No. | AFD- 3F12 |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | Blocking, ELISA, FC |
Species Reactivity | Dog |
Basic Information | |
Specificity | This antibody specifically recognizes canine ICOS, which enhances all basic T-cell responses to a foreign antigen, namely proliferation, secretion of lymphokines, up-regulation of molecules that mediate cell-cell interaction, and effective help for antibody secretion by B-cells. |
Alternative Name | CD278; Inducible T-cell costimulator; Activation-inducible lymphocyte immunomediatory molecule; Inducible co-stimulator; Inducible costimulator; caICOS; canine ICOS; dog ICOS |
UniProt | Q7YR73 |
Immunogen | This antibody was raised by immunizing RBF/DnJ mice with caICOS-murine-IgG2a fusion and hybridomas were generated using accepted methods. |
Application Notes | 3F12 can be used to identify canine ICOS and analyse its activity. The antibody is an antagonist of ICOS and exhibits the ability to significantly suppress mixed leukocyte reactions (MLR) (Sato et al., 2013; pmid: 23694952). Anti-ICOS antibody was also shown to be an effective agent that can be used in the treatment development of Canine Chronic Graft-versus-Host Disease (GVHD) (Graves et al., 2017; pmid: 28958896). |
Antibody First Published | Sato et al. Inducible Costimulator (ICOS) Up-Regulation on Activated T Cells in Chronic Graft-Versus-Host Disease After Dog Leukocyte Antigen-Nonidentical Hematopoietic Cell Transplantation: A Potential Therapeutic Target Transplantation. 2013 Jul 15;96(1):34-41. doi: 10.1097/TP.0b013e318295c025. PMID:23694952 |
Note on publication | This article describes the generation and characterization of the antibody 3F12. |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
