Key features and details | |
Cat. No. | MABL-1867 |
Name | Anti-HLA-G mAbs |
Clone No. | AFD- 9Y5G6 |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | WB, ELISA, IHC |
Species Reactivity | Human |
Basic Information | |
Specificity | This antibody specifically binds the amino acid sequence 'SKEGDGGIMSVRSLSED' of the human HLA-G5 and HLA-G6. HLA-G5 and HLA-G6 are secreted forms of HLA-G that are non-classical major histocompatibility class Ib molecules involved in immune regulatory processes at the maternal-fetal interface. HLA-G expression has been reported in numerous types of cancer, always associated with more advanced stage and aggressive development of the tumor. |
Alternative Name | HLAG; HLA-6.0; HLA-G5; HLAG-6; HLA class I histocompatibility antigen alpha chain G; HLA G antigen; MHC class I antigen G; HLA-G1sol; HLA-G2sol |
UniProt | P17693 |
Immunogen | The original antibody was generated by immunizing BALB/c mice with synthetic human HLA-G molecule heavy chain amino acid sequence '229SKEGDGGIMSVRSLSED319'. |
Application Notes | This antibody can bind human HLA-G peptide 'KEGDGGIMSVRSLSED' in an ELISA with a binding affinity of Ka= 1.5 X 10^(11) L/mol. This antibody was capable of detecting G5 and G6 isoforms of human HLA-G in a western blot and does not cross react with any other isoforms of HLA-G. This antibody can also be used for the immunohistochemical detection of HLA-G5 and HLA-G6 molecules in different gastric cancer tissues using immunohistochemistry (CN113045656). |
Antibody First Published | |
Note on publication | |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
