Anti-HLA-A2/A28 mAbs

Cat. No.

MABL-1855

Name

Anti-HLA-A2/A28 mAbs

Clone No.

AFD- 3PF12

From

Recombinant Antibody

Isotype

Engineer antibody

Application

FC, IF

Species Reactivity

Human

Basic Information

Specificity

Amino acid residues 142-145 (TTKH) of the   alpha2 domain of the heavy chain of the HLA class I molecules are most likely   to be involved in the formation of the epitope recognised by the scfv.

Alternative Name

Human Leukocyte antigen; HLA-A2; HLA-A28;   HLA A2; HLA A28

UniProt

Immunogen

A 57-year-old female presented with post-transfusion   purpura 5 days following a blood transfusion. Serological studies with the   sera of this patient identified the presence of anti-HLA-A2. A 50-ml EDTA   anti-coagulated blood sample was obtained from the patient in the acute phase of her PTP, and mononuclear cells were isolated from the blood sample by   layering onto a Ficoll gradient. An oligo dT primer was used to direct first strand cDNA synthesis and IgG encoded VH was amplified and an scFv library was constructed. Phage particles expressing anti-HLA A2/A28 scFvs were   selected by panning.

Application Notes

ScFv 3PF12 has been used in   immunofluorescence and flow cytometry experiments to test antibody binding to   HLA-A2 positive lymphocytes and platelets (Watkins 2000). The scFv has been   tested in in vitro assays and have been shown to inhibit target cell killing   (Watkins 2003).

Antibody First   Published

Watkins et al. The isolation and characterisation of human monoclonal HLA- A2 antibodies from an immune V gene   phage display library Tissue Antigens 2000:55: 219–228 PMID:10777097

Note on publication

Describes the isolation of scFvs against   HLA-A2/A28 by V gene phage display.

COA Information （For reference only, actual COA shall prevail）

Size

100 μg Purified   antibody.

Concentration

1 mg/ml.

Purification

Protein A affinity   purified

Buffer

PBS with 0.02% Proclin   300.

Concentration

1 mg/ml.

Storage   Recommendation

Store at 4⁰C for up to 3   months. For longer storage, aliquot and store at - 20⁰C.