Anti-HLA-A1:MAGE-A1 complex mAbs

Cat. No.

MABL-1850

Name

Anti-HLA-A1:MAGE-A1 complex mAbs

Clone No.

AFD- HYB3

From

Recombinant Antibody

Isotype

Engineer antibody

Application

functional assay, ELISA, FC

Species Reactivity

Human

Basic Information

Specificity

This antibody is specific for human HLA-A1:MAGE-A1 complex.

Alternative Name

human leukocyte antigen (HLA)/peptide   complex HLA-A1·MAGE-A1; tumor-associated HLA-A1·melanoma-associated antigen   (MAGE)-A1 complex

UniProt

Immunogen

This antibody was raised by panning against   the L chain-shuffling library raised by cloning the G8 VH gene into a vector  containing a library of human Ab kappa and lambda L chains.

Application Notes

The structure of the fab version of this   antibody in complex with HLA-A1 and MAGE-A1 was eludicated using X-ray   crystallography. This was done to determine how the antibody binds to its   ligand (Hülsmeyer et al, 2005; pmid:15537658). To characterize this antibody   flow cytometry was preformed. Flow cytometry was preformed with the fab   version of this antibody on dendritic cells (Chames et al, 2002;   pmid:12097420). To assess the expression of low and high affinity scFv-y CD28   and scFv- receptors on gene-transduced primary human T lymphocytes. Flow   cytometry was preformed on gene-transduced primary human T lymphocytes using   the scFv version of this antibody. Furthermore a cytotoxic assay was   preformed on scFv-y + CD28 or scFv-transduced human T lymphocytes. The   cytotoxic assay was preformed using Cr51 release assay (Willemsen et al,   2005; pmid:15944290). 

Antibody First   Published

Willemsen et al. A major histocompatibility   complex-peptide-restricted antibody and t cell receptor molecules recognize   their target by distinct binding modes: crystal structure of human leukocyte   antigen (HLA)-A1-MAGE-A1 in complex with FAB-HYB3. J Immunol. 2005 Jun   15;174(12):7853-8. PMID:15944290

Note on publication

It was analyzed whether linking the CD28   costimulation structure to it (gamma + CD28) could affect the levels of   MHC-restricted cytolysis and/or cytokine production.

COA Information （For reference only, actual COA shall prevail）

Size

100 μg Purified   antibody.

Concentration

1 mg/ml.

Purification

Protein A affinity   purified

Buffer

PBS with 0.02% Proclin   300.

Concentration

1 mg/ml.

Storage   Recommendation

Store at 4⁰C for up to 3   months. For longer storage, aliquot and store at - 20⁰C.