Anti-HCV core protein mAbs

Cat. No.

MABL-1783

Name

Anti-HCV core protein  mAbs

Clone No.

AFD- 19D9D6

From

Recombinant Antibody

Isotype

Engineer antibody

Application

ELISA

Species Reactivity

HCV, Hepatitis C virus

Basic Information

Specificity

This antibody is specific for the protein   sequence QIVGGVYLL located at residues 29-37 of the HCV core protein.   Hepatitis C virus (HCV) is the primary cause of hepatitis transmitted through   blood transfusion. It is an enveloped virus that contains a single-stranded,   positive-sense RNA genome of approximately 9600 nucleosides. This genome   encodes a polyprotein of 3011 amino acids, which undergoes post-translational   cleavage to form both structural and nonstructural proteins. Among the   structural proteins, the core protein is derived from the amino terminus of   the viral polypeptide (amino acids 1-191).

Alternative Name

Hepatitis C virus core protein

UniProt

Immunogen

The original antibody was generated by   immunizing BALB/c JYco mice with a truncated recombinant protein   corresponding to the immunodominant region (residues 1–120) of the Hepatitis   C Virus (HCV) purified nucleocapsid protein (HCV C120-GST fusion protein).

Application Notes

This antibody was used for an indirect ELISA   to detect anti-HCV core response in human sera, an ELISA competition assays   to assess the interaction between monoclonal antibodies and anticore human   sera, a sandwich ELISA for the capture and detection of viral core antigen in   chronic HCV patient sera, and a sandwich ELISA for the detection of   recombinant core protein (Jolivet-Reynaud et al., 1998; PMID: 9829633). The   crystal structure of the Fab version of this antibody was solved in complex   with an HCV core protein-derived peptide (residues 13-40), revealing the   hydrophobic nature of the recognized epitope (Ménez et al., 2003; PMID:   12574359).

Antibody First   Published

Jolivet-Reynaud et al. HCV core   immunodominant region analysis using mouse monoclonal antibodies and human   sera: characterization of major epitopes useful for antigen detection J Med   Virol. 1998 Dec;56(4):300-9. doi: 10.1002/(sici)1096-9071(199812)56:4300:aid-   jmv3>3.0.co;2-8 PMID:9829633

Note on publication

The original publication focuses on the   generation of monoclonal antibodies (MAbs) against the immunodominant region of the hepatitis C virus (HCV) core protein and the characterization of major   epitopes within this region that are useful for antigen detection.

COA Information （For reference only, actual COA shall prevail）

Size

100 μg Purified   antibody.

Concentration

1 mg/ml.

Purification

Protein A affinity   purified

Buffer

PBS with 0.02% Proclin   300.

Concentration

1 mg/ml.

Storage   Recommendation

Store at 4⁰C for up to 3   months. For longer storage, aliquot and store at - 20⁰C.