Key features and details | |
Cat. No. | MABL-1749 |
Name | Anti-GXM mAbs |
Clone No. | AFD-2E 9 |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | ELISA, IF |
Species Reactivity | Cryptococcus neoformans |
Basic Information | |
Specificity | This antibody is specific for GXM or glucuronoxylomannan from Cryptococcus neoformans. GXM is a polysaccharide expressed on the surface of C. neoformans. This antibody binds an epitope of C. neoformans that is different from the epitope of another anti-GXM antibody 2H1. |
Alternative Name | Glucuronoxylomannan; Cryptococcus neoformans capsular polysaccharide; CNPS |
UniProt | |
Immunogen | The original antibody was generated from the peripheral blood lymphocytes of glucuronoxylomannan-tetanus toxoid conjugate (GXM-TT)-immunized individual. |
Application Notes | This antibody was used for the immunofluorescent labelling of C. neoformans serotype D and A. This antibody can be used in combination with another anti-GXM antibody 2H1 for detection of all four serotypes of C. neoformans GXM using sandwich ELISA. The antibody 2H1 is used as capture antibody and 2E9 is used as a detection antibody (PMID: 7622223). |
Antibody First Published | Pirofski et al. Analysis of human monoclonal antibodies elicited by vaccination with a Cryptococcus neoformans glucuronoxylomannan capsular polysaccharide vaccine. Infect Immun. 1995 Aug;63(8):3005-14. PMID:7622223 |
Note on publication | Describes the generation of two human antibodies from the peripheral blood lymphocytes(PBLs) of a GXMTT-immunized individual. |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
