Key features and details | |
Cat. No. | MABL-1748 |
Name | Anti-GXM mAbs |
Clone No. | AFD- 18B7 |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | functional assay, therapeutic, ELISA, FC, IHC |
Species Reactivity | Cryptococcus neoformans |
Basic Information | |
Specificity | This antibody is specific for GXM from C.neoformans. GXM is a polysacharide expressed on the surface of c.neoformans. |
Alternative Name | Glucuronoxylomannan; Cryptococcus neoformans capsular polysaccharide; CNPS |
UniProt | |
Immunogen | The original mouse version of this antibody was raised by infecting balb/c mice with C. neoformans and then immunizing them with glucuronoxylomannan-tetanus toxoid conjugate (GXM-TT). |
Application Notes | To characterize the mouse version of this antibody an ELISA was performed on native and de-O-acetylated serotype A GMX using the mouse version of this antibody (Casadevall et al, 1992; pmid:1583327). Further immunofluorescence was performed on yeast cells using the mouse version of this antibody (Casadevell et al, 1998; pmid:9624491). Binding of the mouse version of this antibody to normal and C. neoformans-infected tissues was studied by immunohistochemistry (Casadevell et al, 1998; pmid:9624491). The murine macrophage-like cell line J774.16 was used to determine the ability of the mouse version of this antibody to opsonize C. neoformans (Casadevell et al, 1998; pmid:9624491). The effect of the murine version of this antibody on human PMN phagocytosis of C. neoformans was measured by a flow cytometry (Casadevell et al, 1998; pmid:9624491). C3 binding studies were done with formalin- killed cells of strain 388. The kinetics were studied both in the presence and absence of the murine version of this antibody (Casadevell et al, 1998; pmid:9624491). |
Antibody First Published | Casadevall et al. Antibodies elicited by a Cryptococcus neoformans-tetanus toxoid conjugate vaccine have the same specificity as those elicited in infection J Infect Dis. 1992 Jun;165(6):1086-93. PMID:1583327 |
Note on publication | The antibody responses of BALB/c mice to serotype A Cryptococcus neoformans capsular polysaccharide (CNPS) were compared after cryptococcal infection and immunization with a serotype A glucuronoxylomannan-tetanus toxoid conjugate. |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
