Anti-GPIIB/IIIA mAbs

Cat. No.

MABL-1740

Name

Anti-GPIIB/IIIA mAbs

Clone No.

AFD- PDG7

From

Recombinant Antibody

Isotype

Engineer antibody

Application

dot blot, inhibition assays

Species Reactivity

Human

Basic Information

Specificity

PDG7 does not bind to antigen denatured by   heat of pH, indicating the necessity of intact GPIIb/IIIa.

Alternative Name

glycoprotein IIb/IIIa; integrin αIIbβ3;

UniProt

Immunogen

A phagemid library was constructed from the peripheral   blood lymphocytes of a donor who was immunised with RhD+ red blood cells, and   PDG7 was isolated after five rounds of affinity selection.

Application Notes

Dot blots confirmed the specificity of PDG7 for   GPIIB/IIIA. Inhibition assays were used to show that platelet-associated  autoantibodies from AITP patients binds a closely related epitope to that of   the recombinant anti-GPIIb/IIIa phage clones (Escher et al, 1998). Inhibition   studies also confirmed that PDG7 inhibited binding of eluted   platelet-associated autoantibodies from AITP patients to platelets (Escher et   al, 1998).

Antibody First   Published

Escher et al, 1998. Recombinant human   natural autoantibodies against GPIIb/IIIa inhibit binding of autoantibodies   from patients with AITP Br J Haematol. 1998 Aug;102(3):820-8. PMID:9722313

Note on publication

Describes the production of the phage   library and the characterisation of PDG7 through dot blots and inhibition assays.

COA Information （For reference only, actual COA shall prevail）

Size

100 μg Purified   antibody.

Concentration

1 mg/ml.

Purification

Protein A affinity   purified

Buffer

PBS with 0.02% Proclin   300.

Concentration

1 mg/ml.

Storage   Recommendation

Store at 4⁰C for up to 3   months. For longer storage, aliquot and store at - 20⁰C.