Key features and details | |
Cat. No. | MABL-1739 |
Name | Anti-GPIIb/IIIa mAbs |
Clone No. | AFD-10E 5 |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | WB, ELISA, IF |
Species Reactivity | Human |
Basic Information | |
Specificity | The antibody binds to GPIIb/IIIa with an affinity (Kd) of 16.2±6 nM. The antibody completely inhibits platelet aggregation at 7.8 µg/ml. |
Alternative Name | CD41; Integrin alpha-IIb/beta-3; Glycoprotein IIb/IIIa; αIIbβ3 |
UniProt | P08514 |
Immunogen | Human blood platelets. |
Application Notes | The antibody binds specifically to human GPIIb/IIIa, and integrin complex of membrane glycoproteins expressed on the surface of platelets. The GPIIb/IIIa complex acts as a receptor for fibrinogen and Von Willebran factor, hemostatic glycoproteins that mediate platelet aggregation and blood coagulation. The monoclonal antibody 10E5 inhibits the binding of fibrinogen to its platelet receptor, preventing the platelet aggregation, to be used during coronary artery surgeries such as angioplasty. The antibody is also useful in the study of the disease Glanzmann's thrombasthenia, caused by defects in GPIIb/IIIa, and of immune thrombocytopenic purpura, where autoantibodies are produced against GPIIb/IIIa. |
Antibody First Published | Coller BS, Peerschke EI, Scudder LE, Sullivan CA. A murine monoclonal antibody that completely blocks the binding of fibrinogen to platelets produces a thrombasthenic-like state in normal platelets and binds to glycoproteins IIb and/or IIIa. J Clin Invest. 1983 Jul;72(1):325-38. PMID:6308050 |
Note on publication | Describes the development of the monoclonal antibody 10E5, which completely blocks the binding of fibrinogen to platelets. |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
