Anti-gH mAbs

Cat. No.

MABL-1686

Name

Anti-gH mAbs

Clone No.

AFD- YC15

From

Recombinant Antibody

Isotype

Engineer antibody

Application

NTRL, WB, FC, IHC

Species Reactivity

KSHV

Basic Information

Specificity

YC15 was shown to bind ectopically-expressed  gH on transfected cell lines, as well as neutralise infection by KSHV virions   (unpublished observations). Kaposi's sarcoma is a rare type of cancer,   characterised by neoplasmic growth, often associated with AIDS.

Alternative Name

Glycoprotein H; Kaposi's sarcoma-associated herpesvirus glycoprotein H; KSHV glycoprotein H; KSHV gH; KSHV envelope  glycoprotein H; Envelope glycoprotein H

UniProt

F5HAK9

Immunogen

Antibodies were produced from a murine   hybridoma.

Application Notes

The YC15 variable domain sequences were cloned   and redesigned into the scFv format (Cai & Berger, 2011), and further  fused to PE38, a construct of Pseudomonas aeruginosa exotoxin A which is   lacking the N-terminal cell adhesion domain. In this format, YC15 showed   specific dose-dependent binding to surface-expressed gH using flow cytometry.   As an immunotoxin with the fused P. aeruginosa PE38 construct, YC15  facilitated potent cytotoxic activity based on recognition of surface gH epitopes.

Antibody First   Published

Cai & Berger. An immunotoxin targeting   the gH glycoprotein of KSHV for selective killing of cells in the lytic phase   of infection. Antiviral Res. 2011 Jun;90(3): 143-50. PMID: 21440007

Note on publication

Describes the reformatting of YC15 variable   domains (obtained in an unpublished study) as a YC15-scFv-PE38 immunotoxin. The gH-binding properties of YC15 were shown to be retained.

COA Information （For reference only, actual COA shall prevail）

Size

100 μg Purified   antibody.

Concentration

1 mg/ml.

Purification

Protein A affinity   purified

Buffer

PBS with 0.02% Proclin   300.

Concentration

1 mg/ml.

Storage   Recommendation

Store at 4⁰C for up to 3   months. For longer storage, aliquot and store at - 20⁰C.