Key features and details | |
Cat. No. | MABL-1680 |
Name | Anti-GFP mAbs |
Clone No. | AFD- cAbGFP4 |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | crystallization, in vitro, SPR, WB |
Species Reactivity | Aequorea victoria (Water jellyfish, Mesonema victoria) |
Basic Information | |
Specificity | The antibody is specific for green fluorescent protein. The antibody binds wtGFP in a frontwise manner at an exposed loop region between GFP β-strands 6 and 7 as well as parts of β-strand 8. |
Alternative Name | Green fluorescent protein; GFP-Enhancer; anti-GFP-GBP1; GBP1; Enhancer |
UniProt | P42212 |
Immunogen | The original antibody was generated by immunizing alpaca with GFP. A phage display library was constructed and the antibody was selected by panning against GFP. |
Application Notes | The antibody could enhance GFP fluorescenceincrease by a factor of 10 in vitro. A comparable fluorescence modulation was also observed after addition of the antibody to soluble cell extract derived from human embryonic kidney (HEK) 293T cells expressing eGFP. The crystal structure of the GFP–antibody complex was determined. The dissociation constant of the antibody was measured (Kd= 0.59 nM). The fluorescence-enhancement effect induced by binding of GFP to the antibody was used to track inducible translocation of the human estrogen receptor in HeLa-Kyoto cell line (Kirchhofer et al., 2010; PMID： 20010839). In order to improve the affinity for GFP two antibodies that recognize different portions of GFP were linked together. In particular, the antibody was fused together with LaG16 through a (GGGGS)4 linker. |
Antibody First Published | Saerens et al. Identification of a universal VHH framework to graft non-canonical antigen-binding loops of camel single-domain antibodies. J Mol Biol. 2005 Sep 23;352(3): 16095608 |
Note on publication | The original paper describes the generation and characterization of the antibody |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
