Key features and details | |
Cat. No. | MABL-1679 |
Name | Anti-GFP mAbs |
Clone No. | AFD-253 |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | IP, ELISA, IF |
Species Reactivity | Aequorea victoria |
Basic Information | |
Specificity | This antibody binds the green fluorescent protein (GFP) and also cross reacts with derivatives like EGFP, CFP, YFP, BFP and others. |
Alternative Name | Green Fluorescent protein |
UniProt | P42212 |
Immunogen | The original antibody was generated from an alpaca immunized with green fluorescent protein (GFP). |
Application Notes | This chimeric engineered antibody is generated by fusing the original alpaca derived nanobody to mouse IgG1 and rabbit Fc domains to extend the research application of the original. It recognizes GFP in an ELISA. This antibody is recommended for protein visualization using immunofluorescence stainings and immunoprecipitations. It can also be conjugated with fluorophores for direct detection. It can also be immobilized on protein A, beads, resins and plates. The original alpaca VHH antibody is available from ChromoTek. |
Antibody First Published | |
Note on publication | |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
