Key features and details | |
Cat. No. | MABL-1590 |
Name | Anti-Fibrillar alpha-syn mAbs |
Clone No. | AFD- 6E |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | functional assay, ELISA |
Species Reactivity | Human |
Basic Information | |
Specificity | This antibody recognizes the fibrillar morphological form of human alpha-synuclein. Alpha- synuclein is a small 140 amino acid presynaptic neuronal protein, a major component of Lewy bodies and a member of the synuclein family. Misfolding, abnormal accumulation, and secretion of α-Synuclein (α-Syn) are closely associated with synucleinopathies, including Parkinson’s disease (PD). α-syn is abundant in the brain, and smaller amounts are found in the heart, muscles, and other tissues. In the brain, α-syn is found mainly at the tips of neurons in the specialized structures known as presynaptic terminals and has been shown to comprise up to ∼1% of total proteins in the neuronal cytosol. Recent evidence suggests that α- synuclein (α-syn) can contribute to the pathogenesis of amyotrophic lateral sclerosis (ALS). |
Alternative Name | SNCA; NACP; PARK1; alpha-syn; α-Synuclein; α-syn; Non-A beta component of AD amyloid; Non-A4 component of amyloid precursor; fibrillar α-synuclein; fibrillar α-syn |
UniProt | P37840 |
Immunogen | The original antibody was isolated from a human Tomlinson I and J scFv antibody libraries by panning the library against fibrillar α-synuclein prepared as per the method given in PMID：16984950 |
Application Notes | The binding of this antibody to fibrillar alpha-synuclein was confirmed using ELISA. This antibody is not capable of recognizing monomeric and oligomeric forms of alpha-synuclein (PMID： 16984950). |
Antibody First Published | Barkhordarian et al. Isolating recombinant antibodies against specific protein morphologies using atomic force microscopy and phage display technologies. Protein Eng Des Sel. 2006 Nov;19(11)：497-502. PMID：16984950 |
Note on publication | Describes the isolation of antibody fragments that bind to a specific morphology of alpha-synuclein using phage display antibody technology and atomic force microscopy (AFM). |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
