Key features and details | |
Cat. No. | MABL-1578 |
Name | Anti-FGF1 mAbs |
Clone No. | AFD- 1C9 |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | IP, WB, ELISA, IF, IHC |
Species Reactivity | Human |
Basic Information | |
Specificity | This antibody binds the human FGF-1. |
Alternative Name | FGF-1; aFGF; ECGF; HBGF-1; Fibroblast growth factor 1; Acidic fibroblast growth factor; Endothelial cell growth factor; Heparin-binding growth factor 1 |
UniProt | P05230 |
Immunogen | The original antibody was generated by immunizing mice with human FGF-1. |
Application Notes | FGF-1 plays an important role in the regulation of cell survival, cell division, angiogenesis, cell differentiation and cell migration. Studies have shown that FGF-1 is overexpressed in the early stages of several kinds of cancer. Functional analysis showed that the overexpressed scFv1C9 in MCF- 7 cells targeted endogenous FGF-1 and prevented the translocation of FGF-1 into the nucleus, resulting in the blockade of the intracrine pathway of FGF-1, which caused the G1 arrest by p21 up-regulation. Binding of scFv1C9 to FGF-1 was confirmed with ELISA. The antigen-binding ability of scFv1C9 with endogenous FGF- 1 was confirmed in vivo by using of an immunoprecipitation assay. FGF-1 immunohistochemical staining of breast ductal carcinoma tissues was used to demonstrate that FGF-1 was increasingly expressed in breast cancer tissues.(PMID:21360642). |
Antibody First Published | Shi et al. A Novel Single-Chain Variable Fragment Antibody Against FGF-1 Inhibits the Growth of Breast Carcinoma Cells by Blocking the Intracrine Pathway of FGF-1. IUBMB Life (2011); 63(2); 129–137. PMID:21360642 |
Note on publication | Describes the role of anti-FGF1 antibody in blocking the intracrine pathway of FGF-1 and inhibiting growth of breast carcinoma cells. |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
