Anti-EGFR mAbs

Cat. No.

MABL-1473

Name

Anti-EGFR mAbs

Clone No.

AFD-KY303-58

From

Recombinant Antibody

Isotype

Engineer antibody

Application

Blocking, in vitro, in vivo, ELISA

Species Reactivity

Monkey, Human, Mouse

Basic Information

Specificity

This antibody is  specific for human EGFR, but also bind EGFR from mice and monkeys.

Alternative Name

ERBB; ERBB1; HER1; Proto-oncogene c-ErbB-1; Epidermal growth factor receptor; Receptor   tyrosine-protein kinase erbB-1

UniProt

P00533

Immunogen

The original antibody   was raised by immunizing Alpaca with human EGFR antigen.

Application Notes

The original antibody was used for an ELISA on EGFR antigen, it showed that its binding was weaker   than the standard antibody EGFR-BMK2.The ligand blocking activity of this antibody was determined, it was shown that this antibody does not block the binding of EGF to EGFR. The cytotoxicity of this antibody conjugated to MMAE was determined in vitro, it is shown that the antibody drug conjugate is cytotoxic to different tumor cell lines. This antibody was used in vivo on mouse with xenograft tumors made from NCI-H1975 cells, it was shown to effectively inhibit tumor growth (CN117659203B).

Antibody First   Published

Note on publication

COA Information （For reference only, actual COA shall prevail）

Size

100 μg Purified   antibody.

Concentration

1 mg/ml.

Purification

Protein A affinity   purified

Buffer

PBS with 0.02% Proclin  300.

Concentration

1 mg/ml.

Storage   Recommendation

Store at 4⁰C for up to 3   months. For longer storage, aliquot and store at - 20⁰C.