Anti-ectodysplasin A mAbs

Cat. No.

MABL-1463

Name

Anti-ectodysplasin A mAbs

Clone No.

AFD- EctoD2

From

Recombinant Antibody

Isotype

Engineer antibody

Application

SDS-PAGE, WB, Block, ELISA, FC

Species Reactivity

Human, Mouse

Basic Information

Specificity

This antibody recognises the conserved   extracellular, receptor-binding region of murine, avian and human   ectodysplasin-A.

Alternative Name

O54693; Q92838; Ectodysplasin-A; EDA

UniProt

Immunogen

This antibody was raised by immunising Eda-deficient Tabby mice with an active recombinant form of EDA1 (Fc-EDA1).

Application Notes

The specificity of this antibody for EDA has been confirmed in SDS-PAGE, Western blot, ELISA, native gel electrophoresis and FC experiments (Kowalczyk-Quintas et al, 2014). This antibody blocks   mammalian and avian EDA1 and EDA2 from binding EDAR (Kowalczyk-Quintas et al,   2014). When administered to pregnant wild-type mice, this antibody induces   foetal ectodermal dysplasia (Kowalczyk- Quintas et al, 2014).   Therapeutically, this antibody may be useful in the treatment of disorders   arising as a result of excessive EDA1 action.

Antibody First   Published

Kowalczyk-Quintas et al. Generation and   characterization of function- blocking anti-ectodysplasin A (EDA) monoclonal   antibodies that induce ectodermal dysplasia. J Biol Chem. 2014 Feb   14;289(7):4273-85. PMID:24391090

Note on publication

Describes the original generation of this   antibody and its characterisation using SDS- PAGE, WB, ELISA gel   electrophoresis and FC experiments.

COA Information （For reference only, actual COA shall prevail）

Size

100 μg Purified   antibody.

Concentration

1 mg/ml.

Purification

Protein A affinity   purified

Buffer

PBS with 0.02% Proclin   300.

Concentration

1 mg/ml.

Storage   Recommendation

Store at 4⁰C for up to 3   months. For longer storage, aliquot and store at - 20⁰C.