Anti-DNA mAbs

Cat. No.

MABL-1412

Name

Anti-DNA mAbs

Clone No.

AFD- 4B1

From

Recombinant Antibody

Isotype

Engineer antibody

Application

ELISA, IF

Species Reactivity

Species independent

Basic Information

Specificity

This antibody is a polyspecific antibody   that has binding specificity for ssDNA, actin, tubulin, cardiolipin and   laminin.

Alternative Name

ssDNA; actin; tubulin; cardiolipin; laminin.

UniProt

Immunogen

The original antibody was derived from a 12 month old (NZB × NZW)F1 lupus mouse

Application Notes

This antibody is capable of binding ssDNA,   actin, tubulin, cardiolipin and to laminin in a solid phase ELISA. Indirect   immunofluorescence labeling of HEp-2 cells gave a cytoplasmic staining   pattern similar to that obtained with anti-cytoskeleton antibodies. Western   blot analysis demonstrated that mAb 4B1 bore idiotype D23, previously shown   to be characteristic of natural antibodies derived from normal mice. This antibody was able to form glomerular immune deposits (Gilbert et al., 1995;   PMID: 7540257).

Antibody First Published

Gilbert et al. An idiotype D23-bearing   polyspecific, murine anti-DNA monoclonal antibody forms glomerular immune   deposits. Pathogenic role of natural autoantibodies  Mol Immunol. 1995   May;32(7):477-86. PMID:7540257

Note on publication

Describes the generation and characterization of a polyspecific anti-DNA antibody that forms glomerular immune deposits

COA Information （For reference only, actual COA shall prevail）

Size

100 μg Purified antibody.

Concentration

1 mg/ml.

Purification

Protein A affinity purified

Buffer

PBS with 0.02% Proclin 300.

Concentration

1 mg/ml.

Storage Recommendation

Store at 4⁰C for up to 3 months. For   longer storage, aliquot and store at - 20⁰C.