Key features and details | |
Cat. No. | MABL-1413 |
Name | Anti-DNA topoisomerase 1 mAbs |
Clone No. | AFD- 5C2 |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | ELISA |
Species Reactivity | Human |
Basic Information | |
Specificity | This antibody binds human topoisomerase 1. This enzyme controls and alters the topologic states of DNA during transcription. This enzyme catalyzes the transient breaking and rejoining of a single strand of DNA which allows the strands to pass through one another, thus altering the topology of DNA. |
Alternative Name | TOP1; Topo; DNA topoisomerase I |
UniProt | P11387 |
Immunogen | The original antibody was generated by immunizing a barnevelder chicken with an antibody antigen cocktail comprising recombinant purified human antigens annexin XI, CENP-B, Topo, Jo-1, Ro60, Rpp38 and U1A in complete Freund's adjuvant. B cells that were harvested from the bone marrow and peripheral blood lymphocytes of immunized chickens was used to generate recombinant scFv phage display libraries. The specific clone was identified by panning the library against specific antigen. |
Application Notes | The binding characterization of this antibody was done using ELISA (PMID: 18062792). |
Antibody First Published | Hof et al. Multiple-antigen immunization of chickens facilitates the generation of recombinant antibodies to autoantigens. Clin Exp Immunol. 2008 Feb; 151(2): 367–377. PMID: 18062792 |
Note on publication | Describes the generation of chicked antibodies against various human autoantigens. |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
