Anti-DMC1 mAbs

Cat. No.

MABL-1411

Name

Anti-DMC1 mAbs

Clone No.

AFD- 2H12/4

From

Recombinant Antibody

Isotype

Engineer antibody

Application

IP, WB, IF, IHC

Species Reactivity

Cow, Rat, Human, Mouse

Basic Information

Specificity

This antibody is specific for DMC1 and does   not cross-react with the related protein Rad51. DMC1 is a RecA/RAD51 homologue   which is specific for meiosis and critical for meiotic recombination in   Eukaryota.

Alternative Name

Meiotic recombination protein DMC1/LIM15   homolog; DMC1H; LIM15; MGC150473; MGC150472; HsLim15; DMC1 homologue;   disrupted meiotic cDNA1; hDMC1

UniProt

Q14565

Immunogen

This antibody was raised against human DMC1   protein (expressed originally as a 6xHis fusion in E. coli).

Application Notes

IF: 2H12/4 anti-DMC1 antibody was used to   visualise DMC1 on chromatin in I-L2-NS endopolyploid tumour cells in   experiments investigating the role of another protein - REC8 in chromosome   segregation after radiation (Erenpreisa et al., 2009). This antibody was also   utilised to stain paraffin- embedded cervical cancer tissue samples against   DMC1 (Ianzini et al., 2009). IF: Hopkins et al. (2014) successfully used this   antibody to stain chromatin spreads from purified testicular germ cells of   Stag3+/− and Stag3−/− mice for DMC1 in the studies on the role of Stag3. IF:   Another group also used this antibody for the immunofluorescence staining of   spermatocyte chromosome spreads against DMC1 in a study analysing proteins   involved in telomeres' attachment to the nuclear envelope (Wang et al.,   2019).

Antibody First Published

Erenpreisa et al. The role of meiotic   cohesin REC8 in chromosome segregation in γ irradiation-induced endopolyploid   tumour cells Exp Cell Res. 2009 Sep 10;315(15):2593- 603. doi:  10.1016/j.yexcr.2009.05.011. Epub 2009 May   20. PMID: 19463812

Note on publication

This article describes the earliest use of   this anti-DMC1 antibody. It was utilised to visualise DMC1 protein on   chromosomes of endopolyploid tumour cells in experiments investigating the   role of another protein - REC8 in chromosome segregation after radiation.

COA Information （For reference only, actual COA shall prevail）

Size

100 μg Purified antibody.

Concentration

1 mg/ml.

Purification

Protein A affinity purified

Buffer

PBS with 0.02% Proclin 300.

Concentration

1 mg/ml.

Storage Recommendation

Store at 4⁰C for up to 3 months. For   longer storage, aliquot and store at - 20⁰C.