Key features and details | |
Cat. No. | MABL-1407 |
Name | Anti-Dll4 mAbs |
Clone No. | AFD- HMD4-2 |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | Blocking, Functional Studies, FC, IHC |
Species Reactivity | Mouse |
Basic Information | |
Specificity | HMD4-2 recognizes murine Dll4, one of the five major ligands of the Notch signalling pathway. DLL4 signalling has been shown to play a role in the angiogenesis of clear-cell renal tumours, and pancreatic, bladder and colonic cancer. |
Alternative Name | Delta4; Delta-like protein 4; Delta 4; Drosophila Delta homolog 4 |
UniProt | Q9JI71 |
Immunogen | The original hamster IgG version of this antibody was raised by immunizing Armenian hamsters (Oriental Yeast) with a recombinant mouse Dll4 via intraperitoneal injections and subsequent generation of the hybridoma cells. |
Application Notes | This antibody can be used to investigate the role of the signalling pathways related to the Notch receptors and its ligands. The antibody blocks Notch1-Fc and Notch4-Fc binding to mouse Dll4- expressing cells in a dose-dependent manner and does not bind to cells expressing other mouse Notch ligands, such as Dll1, Jagged1, or Jagged2 (Sekine et al., 2009; 19181931). HMD4-2 was demonstrated as an effective infibitor able to neutralize the Dll4-dependent Notch signalling (Sekine et al., 2009; 19181931). It was used to block Dll4-mediated Notch signaling in LDL-receptor–deficient (Ldlr−/−) mice fed a high-fat, high-cholesterol diet, a model that produces atherosclerosis and metabolic disturbances resembling those seen in the cardiometabolic syndrome in order to test the hypothesis in vivo that Dll4 contributes to the pathogenesis of the cardiometabolic syndrome (Fukuda et al., 2012; pmid: 22699504). |
Antibody First Published | Moriyama et al. Delta-like 1 is essential for the maintenance of marginal zone B cells in normal mice but not in autoimmune mice Int Immunol. 2008 Jun;20(6):763-73. doi: 10.1093/intimm/dxn034. Epub 2008 Apr 1. PMID:18381350 |
Note on publication | Describes the generation and characterization of the HMD4-2 antibody. |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
