Key features and details | |
Cat. No. | MABL-1406 |
Name | Anti-DLL4 mAbs |
Clone No. | AFD- 61B |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | Blocking, ELISA, IF, IHC |
Species Reactivity | Human |
Basic Information | |
Specificity | This antibody is specific for the EGF-like 3 domain of Dll4. |
Alternative Name | Delta-like protein 4; Drosophila Delta homolog 4; Delta4 |
UniProt | Q9NR61 |
Immunogen | The original antibody was generated by immunizing mice with soluble human Dll4-His protein. |
Application Notes | U87MG and HT29 cells were immune-stained with this antibody (Zhou et al, 2015; pmid:26288060). This antibody is used for immunohistochemistry on U87MG and HT29 (Zhou et al, 2015; pmid: 26288060). The 64Cu-chelator DOTA conjugated version of this antibody was used for the positron- emission tomography (PET) imaging of human glioblastoma and colorectal cancer xenografts (Zhou et al, 2015; pmid:26288060). This antibody was used for immunohistochemistry on spheroids and monolayers cells (US7906116). ELISA was performed with this antibody on Dll4 truncation mutants (US7906116). This antibody is able to block the Dll4-Notch1 interaction (US7906116). |
Antibody First Published | Zhou et al. PET Imaging of Dll4 Expression in Glioblastoma and Colorectal Cancer Xenografts Using (64)Cu-Labeled Monoclonal Antibody 61B. Mol Pharm. 2015 Oct 5;12(10):3527-34. PMID: 26288060 |
Note on publication | A (64)Cu-labeled probe for positron emission tomography (PET) imaging of tumor Dll4 expression was developed. |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
