Key features and details | |
Cat. No. | MABL-1378 |
Name | Anti-desmosome/hemidesmosome mAbs |
Clone No. | AFD- F12 |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | IEM, IF |
Species Reactivity | Human |
Basic Information | |
Specificity | F12 recognizes an unknown polypeptide of desmosomal and hemidesmosomal plaques with a MW of 185 kDa (determined from immunoblotting using bovine tongue epithelium extract as the substrate), and by IEM (immunoelectron microscopy) it was determined that it is the intracellular part of the desmosome that is bound (Gilbert, 1997). F12 shares some immunochemical properties with autoantibodies present in paraneoplastic pemphigus sera. Pemphigus diseases are those resulting in blistering of the skin due to the production of autoantibodies against keratinocyte surface proteins - autoantibodies of patients with pemphius vulgaris recognize desmoglein 1 (Dsg1), a major glycoprotein of the desmosome complex. |
Alternative Name | |
UniProt | |
Immunogen | F12 was established by fusing peripheral blood lymphocytes derived from a human vulgaris patient with a hetermyeloma cell line. |
Application Notes | F12 labels the keratinocyte membrane and the basement membrane zone of rat tongue sections by indirect immunofluorescence (IIF). The antibody also stains both the cell membrane and the basement membrane zone of stratified squamous epithelia, and other epithelial tissues such as urinary bladder, small bowel, thymus, and liver. Non-epithelial tissues, such as myocardium are additionally stained. Sera from patients with pemphigus vulgaris can be incubated with human skin sections to determine their capacity to block the binding of F12 in an IIF blocking assay. IEM (immunoelectron microscopy) can be used to determine the ultralocalization of the antibody. |
Antibody First Published | Gilbert et al. Production of a Human Monoclonal Anti-epithelial Cell Surface Antibody Derived from a Patient with Pemphigus Vulgaris Journal of Autoimmunity (1992) 5,173-182 PMID:1627232 |
Note on publication | Describes the generation of an anti-desmosome IgM antibody which recognises a 185 kDa protein which was also recognized by the sera of two patients with pemphigus vulgaris. Its binding properties were studied. |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
