Key features and details | |
Cat. No. | MABL-1377 |
Name | Anti-Desmoglein-3 mAbs |
Clone No. | AFD- F779 |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | ELISA, IF |
Species Reactivity | Human |
Basic Information | |
Specificity | This antibody binds human desmoglein-3 between amino acids 1-161 and does not cross react with desmoglein-1. It is a component of intercellular desmosome junctions. It is involved in the interaction of plaque proteins and intermediate filaments mediating cell-cell adhesion. Pemphigus vulgaris (PV) is a potentially lethal skin disease in which epidermal blisters occur as the result of the loss of cell-cell adhesion caused by the action of autoantibodies against desmoglein 3. It is characterized by suprabasal blistering of skin and mucous membranes. |
Alternative Name | DSG3; PVA; CDHF6; Desmoglein 3; 130 kDa pemphigus vulgaris antigen; Cadherin family member 6 |
UniProt | P32926 |
Immunogen | The original antibody was generated from the peripheral blood of four patients with active untreated pemphigus vulgaris (PV) using heterohybridoma techniques. |
Application Notes | This antibody can be used for the determination of desmoglein-3 using ELISA. The binding affinity of IgG1 version of this antibody for human desmoglein-3 was determined using surface plasmon resonance and was found to be Kd = 1.77 nM (PMID: 24942562). This antibody was also used in the direct and indirect immunofluorescence staining of frozen human skin sections (PMID: 24942562; 27304671) The IgG1 and IgG4 variants of this antibody induce loss of cell surface Dsg3 in primary human epidermal keratinocytes and suprabasal blisters in human skin (PMID: 27304671). This antibody is pathogenic in nature and caused suprabasal blisters in human skin at comparable doses of 25 μg and 50 μg (PMID: 27304671). |
Antibody First Published | Cho et al. Shared VH1-46 gene usage by pemphigus vulgaris autoantibodies indicates common humoral immune responses among patients. Nat Commun. 2014 Jun 19;5:4167. PMID: 24942562 |
Note on publication | This paper identifies VH1-46 autoantibody gene as a common gene expressed in patients with pemphigus vulgaris. |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
