Key features and details | |
Cat. No. | MABL-1343 |
Name | Anti-Cytokeratin 5 and 8 mAbs |
Clone No. | AFD- RCK102 |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | WB, FC, IHC |
Species Reactivity | canine, Feline, Hamster, Rabbit, Rat, Swine, Human, Mouse |
Basic Information | |
Specificity | The original antibody binds to Cytokeratin 5 and 8. |
Alternative Name | K5; CK-8; Keratin K5 + K8; Keratin, type II cytoskeletal 8; Cytokeratin-8; Keratin-8; K8; Type-II keratin Kb8; Keratin, type II cytoskeletal 5; 58 kDa cytokeratin; Cytokeratin-5; CK-5; Keratin-5; Type-II keratin Kb5 |
UniProt | P05787; P13647 |
Immunogen | The original antibody was generated by immunizing a mouse with cytokeratins from a Human lung cancer cell line (MR21). |
Application Notes | The antibody recognized keratins 5 and 8, and as a result stained virtually all epithelial tissues. This antibody was used for immunohistochemistry on classic SCLC as well as the adenocarcinoma cell lines (Broers et al., 1986; PMID: 2433295). Further, endocervical columnar cells, cervical immature squamous metaplasia, cervical CIN I, CIN II and CIN III reacted with the antibody in immunohistochemistry (Smedts et al., 1990; PMID: 1690513). The antibody detected keratins 5 and 8 by western blot analysis on T 24, HeLa and KB cells (Ramaekers et al., 1987; PMID: 2436934). |
Antibody First Published | Broers et al. Intermediate filament proteins in classic and variant types of small cell lung carcinoma cell lines: a biochemical and immunochemical analysis using a panel of monoclonal and polyclonal antibodies J Cell Sci. 1986 Jul;83:37-60. doi: 10.1242/jcs.83.1.37. PMID: 2433295 |
Note on publication | The original paper describes the generation and characterization of the antibody. |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
