Key features and details | |
Cat. No. | MABL-1342 |
Name | Anti-Cytokeratin 20 mAbs |
Clone No. | AFD- 10F6B12A9 |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | WB, ELISA, IHC |
Species Reactivity | Human |
Basic Information | |
Specificity | This antibody is specific for amino acids 411-424 of human cytokeratin 20. Cytokeratins are intermediate filaments found in the intracytoplasmic cytoskeleton of epithelial tissue. There are 19 keratin polypeptides are found in human epithelium. Cytokeratin 20 plays a significant role in maintaining keratin filament organization in intestinal epithelia. When phosphorylated, it plays a role in the secretion of mucin in the small intestine. |
Alternative Name | CK20; CK-20; K20; KRT20; Cytokeratin-20; Keratin-20; Keratin type I cytoskeletal 20 ;Protein IT; CGMCC No 16204; Ks20.8 |
UniProt | P35900 |
Immunogen | The original antibody was generated by immunizing female ICR mice with human CK20 peptide (amino acids 411-424) conjugated to KLH in equal volume of Freund's incomplete adjuvant. |
Application Notes | This antibody can bind human cytokeratin 20 in an ELISA. It is also capable of detecting human cytokeratin 20 in a western blot assay. This antibody is similar to commercially available anti-Cytokeratin 20 antibody KS20.8 in staining various human tissues. This antibody was used in the immunohistochemical staining of paraffin sections of stomach, colon and colonic cancer tissues (CN109734805). |
Antibody First Published | |
Note on publication | |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
