Anti-Cytokeratin 5/6 mAbs

Cat. No.

MABL-1344

Name

Anti-Cytokeratin 5/6 mAbs

Clone No.

AFD- AbM58019-7H4

From

Recombinant Antibody

Isotype

Engineer antibody

Application

ELISA, IHC

Species Reactivity

Human

Basic Information

Specificity

This antibody is capable of binding human   cytokeratin 5 and human cytokeratin 6. Cytokeratins are intermediate   filaments found in the intracytoplasmic cytoskeleton of epithelial tissue.   Cytokeratin 5/6 is often expressed in a basal cell layer of normal mammary   ducts. Studies have also shown that CK5/6 can be used as a characteristic   marker of basal-like breast cancer.

Alternative Name

CK5/6; CK5; CK-5; K5 KRT5; Keratin, type II   cytoskeletal 5; cytokeratin-5; 58 kDa cytokeratin; Type-II keratin Kb5; CGMCC   NO 21982; D5/16B4

UniProt

P13647

Immunogen

The original antibody was generated by   immunizing 4-6 weeks old female ICR mice with recombinant protein comprising amino   acids 488-590 of cytokeratin 5 in equal volume of Freund's incomplete   adjuvant.

Application Notes

The binding characterization of this   antibody was done using ELISA. The original IgG2b antibody binds cytokeratin   5/6 with a high affinity constant of 3.84 × 10^(9) M^(-1). This antibody was   also used in the immunohistochemical analysis of esophagus and esophageal   squamous cell carcinoma. The immunohistochemical staining pattern of this   antibody is similar to commercially available antibody D5/16B4 (CN113845592).

Antibody First Published

Note on publication

COA Information （For reference only, actual COA shall prevail）

Size

100 μg Purified antibody.

Concentration

1 mg/ml.

Purification

Protein A affinity purified

Buffer

PBS with 0.02% Proclin 300.

Concentration

1 mg/ml.

Storage Recommendation

Store at 4⁰C for up to 3 months. For   longer storage, aliquot and store at - 20⁰C.