Anti-CTLA-4 mAbs

Cat. No.

MABL-1287

Name

Anti-CTLA-4 mAbs

Clone No.

AFD- 9H10

From

Recombinant Antibody

Isotype

Engineer antibody

Application

Neutralisation, proliferation assays, WB,   ELISA, FC

Species Reactivity

Mouse

Basic Information

Specificity

This antibody is specific for murine CTLA-4,   an inhibitory receptor that acts as the primary negative regulator of T-cell   responses. CTLA-4 is expressed predominantly by activated T cells, with   significantly higher levels of expression on CD8+ T cells than CD4+ T cells.

Alternative Name

CD152; CTLA4; cytotoxic T-lymphocyte-associated antigen 4; Cytotoxic T- lymphocyte protein 4;

UniProt

P09793

Immunogen

This antibody was raised by immunising   Syrian hamsters with Staphylococcus A bacteria coated in CTLA-4, isolating B   cells from the immunised hamsters and fusing these with the P3X3.Ag8.653   myeloma line to produce stable hybridomas.

Application Notes

CTLA-4 is upregulated on T cells following   their activation, and acts as a negative regulator of T cell responses;   CTLA-4 binds to the B7 molecules CD80 and 86, resulting in the delivery of an   inhibitory signal, and consequent downregulation of T cell-mediated immunity.   Administration of 9H10 blocks the interaction between CTLA-4 on the T cell   surface and CD80 and CD86. This promotes the activation of effector T cells   and stimulates the immune response raised against weak antigens, including   tumour antigens. While this antibody alone does not enhance T cell   proliferation, it does significantly increase T cell proliferation when   administered together with anti-CD28 (clone 37.51) (Krummel & Allison,   1995), anti-OX40 and anti-GITR (Houot & Levy, 2009). Blocking CTLA-4   induces T cell anti-tumour immunity in animal models, both by suppressing   regulatory T cell activity and directly promoting CD8+ T cell effector   function (Peggs et al, 2009).

Antibody First Published

Krummel & Allison CD28 and CTLA-4 Have   Opposing Effects on the Response of T cells to Stimulation J Exp Med. 1995   Aug 1; 182(2): 459–465. PMID:7543139

Note on publication

Describes the original generation and characterisation of this antibody.

COA Information （For reference only, actual COA shall prevail）

Size

100 μg Purified antibody.

Concentration

1 mg/ml.

Purification

Protein A affinity purified

Buffer

PBS with 0.02% Proclin 300.

Concentration

1 mg/ml.

Storage Recommendation

Store at 4⁰C for up to 3 months. For   longer storage, aliquot and store at - 20⁰C.