Key features and details | |
Cat. No. | MABL-1286 |
Name | Anti-CTLA-4 mAbs |
Clone No. | AFD- 9D9 |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | Block, IF |
Species Reactivity | Mouse |
Basic Information | |
Specificity | The antibody is specific for CTLA-4. |
Alternative Name | CD152; CTLA4; Cytotoxic T-Lymphocyte Associated Protein 4; Insulin- Dependent Diabetes Mellitus 12; Celiac Disease 3; Ligand And Transmembrane Spliced Cytotoxic T Lymphocyte Associated Antigen 4; Cytotoxic T Lymphocyte Associated Antigen 4 Short Spliced Fo |
UniProt | P09793 |
Immunogen | The antibody was raised against CTLA-4. |
Application Notes | The antibody has been used to block CTLA-4 and has been shown to enhance T-cell immunity against tumours (Quezada et al, 2006). 9D9 also has an anti-B16/BL6 melanoma effect in non- irradiated tumour-bearing recipients when given as combinatorial therapy with Gvax (Peggs et al, 2009). Selby et al. (PMID: 24777248) show how conversion of the original mouse IgG2b to a mouse IgG2a greatly increases anti-tumor activity of the 9D9 antibody in both MC38 (C57BL/6 mice) and CT26 (BALB/c mice) models. This mechanism both involves blockade of CTLA4 as well as depletion of tumor-infiltrating regulatory T-cells. Fc Silent antibodies were shown to have reduced anti-tumor activity as they did not deplete tumor infiltrating regulatory T-cells, however they lead to expansion of peripheral Tregs. |
Antibody First Published | Simpson T et al. Fc-dependent depletion of tumor-infiltrating regulatory T cells co-defines the efficacy of anti–CTLA-4 therapy against melanoma J Exp Med. 2013 Aug 26; 210(9): 1695–1710. PMID:23897981 |
Note on publication | Describes the characterisation of the Fcγ receptor-dependent activity of 9D9 on the T reg cell compartment. |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
