Key features and details | |
Cat. No. | MABL-1288 |
Name | Anti-CTLA-4 mAbs |
Clone No. | AFD- CP-675,206 (Tremelimumab, 11.2.1) |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | FC, Block, ELISA |
Species Reactivity | Human, Cynomolgus Monkey |
Basic Information | |
Specificity | This antibody binds human and cynomolgus monkey CTLA4 and does not cross react with mouse, hamster, rat, or rabbit CTLA4. |
Alternative Name | CD152; CTLA4; Cytotoxic T-lymphocyte protein; Cytotoxic T-lymphocyte- associated antigen 4 |
UniProt | P16410 |
Immunogen | The original antibody was generated by immunizing XenoMouse® mice with cells expressing human CTLA-4 recombinantly. |
Application Notes | Formerly known as ticilimumab, this anitbody is a fully human anti-huCTLA4 antagonist. It binds human and cycynomolgus monkey CTLA4 in flow cytometric analysis. It binds immobilized human and monkey CTLA4-Ig with average affinities of 0.28 and 0.98 nM, respectively. It is also reported that this antibody does not induce release of cytokines and also does not bind to human leukocyte Fc receptors. It blocks binding of human CTLA4-Ig to immobilized B7.1 and B7.2 with average IC50 values of 0.65 and 0.50 nM in competition binding studies. It was reported to enhance IL-2 and interferon-gamma production in human T cell blasts stimulated with B7-positive Raji cells and also enhanced IL-2 production in a concentration dependent manner in human and cynomolgus monkey whole blood (WB) and peripheral blood mononuclear cells (PBMC) stimulated with staphylococcal enterotoxin A (SEA) superantigen (Hansen et al, 2004). |
Antibody First Published | Hanson et al. Preclinical in vitro characterization of anti-CTLA4 therapeutic antibody CP-675,206. Cancer Research, Volume: 64, Page: 877-877. PMID |
Note on publication | Describes the in vitro characterization of this antibody. |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
