Key features and details | |
Cat. No. | MABL-1285 |
Name | Anti-CTLA-4 mAbs |
Clone No. | AFD-5E 3 |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | in vivo, ELISA |
Species Reactivity | Human |
Basic Information | |
Specificity | The antibody is specific for CTLA-4. CTLA-4 is a protein receptor that functions as an immune checkpoint and downregulates immune responses. |
Alternative Name | Cytotoxic T-lymphocyte protein 4; Cytotoxic T-lymphocyte-associated antigen 4; CD152 |
UniProt | P16410 |
Immunogen | The original antibody was generated by immunizing BALB/c mice with CTLA-4 protein. |
Application Notes | The specificity of the antibody was confirmed by ELISA analysis. The kinetic parameters of human CTLA-4 protein binding to the antibody were measured and they resulted to be at the sub-nm level. The antitumor activity of the humanized antibody was demonstrated in vivo in animal models. It was proved that the antibody inhibited the tumor in a dose-dependent manner. Mouse PD-1 antibody was combined with the antibody and used to evaluate the toxicity to mice; these experiments compared with the use of Ipilimumab in combination with PD-1 antibody. Results showed that after 5E3 antibody was used in combination with PD-1 antibody, the immunity to the body and the toxicity of heart, liver and lung are significantly lower than the toxicity of Ipilimumab in combination with PD-1 antibody (CN110343180). |
Antibody First Published | |
Note on publication | |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
